Biochemistry Department

Daniel Gallie


  Dr. Daniel Gallie

Daniel R. Gallie
Professor of Biochemistry

Plant Biochemistry
Molecular Biology

Gallie Research Group
PubMed Citations


Awards and Honors
  • 2005 American Association for the Advancement of Science, Biological Sciences
Selected Publications
  • Gallie, D.R., Le, H.,Caldwell, C., Tanguay, R.L., Hoang, N.X., and Browning, K.S. 1997. The phosphorylation state of translation initiation factors is regulated developmentally and following heat shock in wheat.  J. Biol. Chem. 272: 1046-1053.
  • Le, H., Chang, S.-C., Tanguay, R.L., and Gallie, D.R.  1997. The wheat poly(A)-binding protein functionally complements
    Pab1p in yeast.   Eur. J. Biochem. 243: 350-357.
  • Chang, S.-C., and Gallie, D.R.  1997. RNase activity decreases following a heat shock in wheat leaves and correlates with its posttranscriptional modification.  Plant Physiol. 113: 1253-1263.
  • Le, H., Tanguay, R.L., Balasta, M.L., Wei, C.-C., Browning, K.S., Metz, A.M., Goss, D.J., and  Gallie, D.R. 1997.  Translation initiation factors eIF-iso4G and eIF-4B interact with the poly(A)-binding protein and increase its RNA binding activity. J. Biol Chem. 272: 16247-16255.
  • Smith, B.L., Gallie, D.R., Le, H., and Hansma, P.K. 1997. Visualization of poly(A)-binding protein complex formation with poly(A) RNA using atomic force microscopy.  J. Struc. Biol. 119: 109-117.
  • Young, T.E., Gallie, D.R. and DeMason, D.A. 1997. Ethylene mediated programmed cell death during maize endosperm development of Su and sh2 genotypes.  Plant Physiol. 115: 737-751.
  • Gallie, D.R. and Chang, S.-C. 1997. Signal transduction in the carnivorous plant Sarracenia purpurea: regulation of secretory hydrolase expression during development and in response to resources.  Plant Physiol. 115: 1461-1471.
  • Gallie, D.R., Le, H.,Caldwell, C., and Browning, K.S.  1998. Analysis of translation elongation factors from wheat during development and following heat shock. Biochem. Biophys. Res. Comm. 245: 295-300.
  • Gallie, D.R., Le, H., Tanguay, R.L., and Browning, K.S. 1998. Translation initiation factors are differentially regulated in cereals during development and following heat shock. Plant J. 14: 715-722.
  • Le, H., Browning, K.S., and  Gallie, D.R. 1998. The phosphorylation state of the wheat translation initiation factors eIF4B, eIF4A, and eIF2 is differentially regulated during seed development and germination. J. Biol Chem. 273: 20084-20089.
  • Wells, D.R., Tanguay, R.L., Le, H., and  Gallie, D.R. 1998. HSP101 functions as a specific translational regulatory protein whose activity is regulated by nutrient status. Genes & Devel. 12: 3236-3251.
  • Young, T.E., and Gallie, D.R. 1999. Analysis of programmed cell death in wheat endosperm reveals differences in endosperm development between cereals.  Plant Mol. Biol. 39: 915-926.
  • Niepel, M., and Gallie, D.R.  1999. Identification and characterization of the functional elements within the tobacco etch viral 5'-leader required for cap-independent translation. J. Virol. 73: 9080-9088.
  • Niepel, M., Ling, J. and Gallie, D.R. 1999. The effect of secondary structure on the functional interaction between the 5'-cap and the poly(A) tail during translation. FEBS Lett. 462: 79-84.
  • Le, H. and Gallie, D.R. 2000. Sequence diversity and conservation of the poly(A)-binding protein in plants. Plant Sci. 152: 101-114.
  • Young, T.E., and Gallie, D.R.  2000. Regulation of programmed cell death in maize endosperm by abscisic acid.  Plant Mol. Biol. 42: 397-414.
  • Le H., Browning, K.S., and Gallie, D.R. 2000. The phosphorylation state of poly(A)‑binding protein specifies its binding to poly(A) RNA and its interaction with eukaryotic initiation factor (eIF) 4F, eIFiso4F, and eIF4B.  J. Biol. Chem. 275: 17452‑17462.
  • Ling, J., Wells, D.R., Tanguay, R.L., Dickey, L.F., Thompson, W.F., and Gallie, D.R.  2000. Heat shock protein HSP101 binds to the Fed‑1 internal light regulatory element and mediates its high translational activity. Plant Cell 12: 1213‑1228.
  • Gallie, D.R., Ling, J., Niepel, M., Morley, S.J., and Pain, V.M. 2000. The role of 5'‑leader length, secondary structure and PABP concentration on cap and poly(A) tail function during translation in Xenopus oocytes.  Nucl. Acids Res. 28: 2943‑2953.
  • Burks, E.A., Bezerra, P.P., Le, H., Gallie, D.R., and Browning, K.S. 2001. Plant initiation factor 3 subunit composition resembles mammalian initiation factor 3 and has a novel subunit. J. Biol. Chem. 276: 2122-2131.
  • Gallie, D.R. and Browning, K.S. 2001. eIF4G functionally differs from eIFiso4G in promoting internal initiation, cap‑independent translation, and translation of structured mRNAs.  J. Biol. Chem.  276: 36951‑36960.
  • Young, T.E., Ling, J., Geisler-Lee, J., Tanguay, R.L., Caldwell, C., and Gallie, D.R.  2001. Developmental and thermal regulation of the maize heat shock protein, HSP101.  Plant Physiol. 127: 777‑791.
  • Gallie, D.R. 2001. Cap-independent translation conferred by the 5'-leader of tobacco etch virus is eIF4G-dependent. J. Virol. 75: 12141‑12152.
  • Gallie, D.R., Chang, S-C., Young. T.E. 2002. Induction of RNase and nuclease activity in cultured maize endosperm cells following sucrose starvation. Plant Cell, Tissue, Organ Culture 68: 163‑170.
  • Dhaubhadel, S., Browning, K.S., Gallie, D.R., Krishna, P. 2002. Brassinosteroid functions to protect the translational machinery and heat‑shock protein synthesis following thermal stress.  Plant J.  29: 681‑691.
  • Gallie DR.  2002. The 5'‑leader of tobacco mosaic virus promotes translation through enhanced recruitment of eIF4F. Nucl. Acids Res. 30: 3401‑311.
  • Gallie, D.R., Fortner, D., Peng, J., and Puthoff, D. 2002. ATP‑dependent hexameric assembly of the heat shock protein, Hsp101, involves multiple interaction domains and a functional C‑proximal nucleotide‑binding domain. J. Biol. Chem. 277: 39617‑39626.
  • Ling, J., Morley, S.J., Pain, V.M., Marzluff, W.F., and Gallie, D.R.  2002. The histone 3'-terminal stem loop binding protein enhances translation through a functional and physical interaction with eIF4G and eIF3. Mol. Cell. Biol. 22: 7853‑7867.
  • Agarwal, M., Sahi, C., Katiyar-Agarwal, S., Agarwal, S., Young, T., Gallie, D.R., Sharma, V.M., Ganesan, K., Grover, A. 2003. Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types. Plant Mol Biol. 51: 543-553.
  • Chen, Z., Young, T.E., Ling, J., Chang, S.C., Gallie, D.R. 2003. Increasing vitamin C content of plants through enhanced ascorbate recycling. Proc. Natl. Acad. Sci. U.S.A. 100: 3525-3530.
  • Young, T.E., and Gallie, D.R. 2004. The ethylene biosynthetic and perception machinery is differentially expressed during maize endosperm and embryo development. Mol. Gen. Genomics 271: 267-281.
  • Chen, Z., and Gallie, D.R. 2004. The ascorbic acid redox state controls guard cell signaling and stomatal movement. Plant Cell 16: 1143-1162.
  • Young, T.E., Giesler-Lee, J., and Gallie, D.R. 2004. Senescence induced expression of cytokinin reverses pistil abortion during maize flower development. Plant J. 38: 910-922.
  • Chen, Z., Ling, J., and Gallie, D.R. 2004. RNase activity requires formation of disulfide bonds and is regulated by the redox state. Plant Mol. Biol.  55: 83-96.
  • Young, T.E., Meeley, R.B., and Gallie, D.R. 2004. ACC synthase expression regulates leaf performance and drought tolerance in maize. Plant J.  40: 813-825.
  • Gallie D.R. 2004. The role of the initiation surveillance complex in promoting efficient protein synthesis. Biochem Soc Trans. 32: 585-588.
  • Krab, I.M., Gallie, D.R., and Bol, J.F. 2005. Coat protein enhances translational efficiency of Alfalfa mosaic virus RNAs and interacts with the eIF4G component of initiation factor eIF4F. J. Gen. Virol. 86: 1841-1849.
  • Chen, Z., and Gallie, D.R.  2005. Increasing tolerance to ozone by elevating foliar ascorbic acid confers greater protection against ozone than does increasing avoidance.  Plant Physiol. 138: 1673-1689.
  • Zeenko, V, and Gallie, D.R.  2005. Cap-independent translation of tobacco etch virus is conferred by an RNA pseudoknot in the 5' leader.  J. Biol. Chem. 280: 26813-26824.
  • Geisler-Lee, J., and Gallie, D.R. 2005. Aleurone cell identity is suppressed following connation in maize kernels. Plant Physiol. 139: 204-212
  • Cheng S., and Gallie D.R. 2006. Wheat eukaryotic initiation factor 4B organizes assembly of RNA and eIFiso4G, eIF4A, and poly(A)-binding protein. J. Biol. Chem 281: 24351-24364.
  • Chen, Z., and Gallie, D.R. 2006. Dehydroascorbate reductase affects leaf growth, development, and function. Plant Physiol. 142: 775-787.
  • Ray, S., Yumak, H., Domashevskiy, A., Khan, M.A., Gallie, D.R., and Goss, D.J. 2006. Tobacco etch virus mRNA preferentially binds wheat germ eukaryotic initiation factor  (eIF) 4G rather than eIFiso4G. J. Biol. Chem. 281: 35826-35834.
  • Siddiqui, N., Osborne, M.J., Gallie, D.R., and Gehring, K. 2007. Solution structure of the PABC domain from wheat poly (A)-binding protein: an insight into RNA metabolic and translational control in plants. Biochemistry 46: 4221-4231.
  • Cheng, S., Gallie, D.R.  2007. eIF4G, eIFiso4G, and eIF4B bind the poly(A)-binding protein through overlapping sites within the RNA recognition motif domains. J. Biol. Chem. 282: 25247-25258.
  • Cheng, S., Sultana, S., Goss, D.J. and Gallie DR. 2008. Translation initiation factor 4B homodimerization, RNA binding, and interaction with poly(A)-binding protein are enhanced by zinc. J. Biol. Chem. 283:36140-36153.
  • Khan, M.A., Yumak, H., Gallie, D.R., and Goss, D.J. 2008. Effects of poly(A)-binding protein on the interactions of translation initiation factor eIF4F and eIF4F.4B with internal ribosome entry site (IRES) of tobacco etch virus RNA. Biochim. Biophys. Acta. 1779:622-627.
  • Chen, Z., and Gallie, D.R. 2008. Dehydroascorbate reductase affects non-photochemical quenching and photosynthetic performance. J. Biol. Chem. 283:21347-21361.
  • Liu, Z., Cheng, S., Gallie, D.R., and Julian, R.R. 2008. Exploring the mechanism of selective noncovalent adduct protein probing mass spectrometry utilizing site-directed mutagenesis to examine ubiquitin. Anal. Chem. 80:3846-3852.
  • Khan, M.A., Miyoshi, H., Gallie, D.R., and Goss, D.J. 2008. Potyvirus genome-linked protein, VPg, directly affects wheat germ in vitro translation: interactions with translation initiation factors eIF4F and eIFiso4F. J. Biol. Chem. 283:1340-1349.
  • Gallie DR, Geisler-Lee J, Chen J, Jolley B. 2009.Tissue-specific expression of the ethylene biosynthetic machinery regulates root growth in maize. Plant Mol. Biol. 69: 195-211.
  • Geisler-Lee J, Caldwell C, and Gallie DR. 2010. Expression of the ethylene biosynthetic machinery in maize roots is regulated in response to hypoxia. J. Exp. Bot. 61: 857-871.

  • Gallie   DR. 2010. Regulated ethylene insensitivity through the inducible expression of the Arabidopsis etr1-1 mutant ethylene receptor in tomato. Plant Physiol. 152: 1928-1939.

  • Lellis AD, Allen ML, Aertker AW, Tran JK, Hillis DM, HarbinCR, Caldwell C, Gallie DR, and Browning KS. 2010. Deletion of the eIFiso4G subunit of the Arabidopsis eIFiso4F translation initiation complex impairs health and viability. Plant Mol. Biol. 74: 249-263.

  • Chen, J.F., and Gallie, D.R. 2010. Analysis of the functional conservation of ethylene receptors between maize and Arabidopsis. Plant Mol. Biol. 74: 405-421.

  • Cheng, S., and Gallie, D.R. 2010. Competitive and non-competitive binding of eIF4B, eIF4A, and the poly(A)-binding protein to wheat translation initiation factor eIFiso4G. Biochemistry 49: 8251-8265.

  • Chen, Z., and Gallie, D.R. 2012. Induction of monozygotic twinning by ascorbic Acid in tobacco. PLoS One.7: e39147.

  • Chen, Z., and Gallie, D.R. 2012. Violaxanthin de-epoxidase is rate-limiting for non-photochemical quenching under subsaturating light or during chilling in Arabidopsis. Plant Physiol Biochem. 58C: 66-82.

  • Botanga, C.J., Bethke, G., Chen, Z., Gallie, D.R., Fiehn, O., Glazebrook, J. 2012. Metabolite profiling of Arabidopsis inoculated with Alternaria brassicicola reveals that ascorbate reduces disease severity. Mol. Plant Microbe Interact. 25: 1628-1638. PMID: 23134520.

  • Gallie, D.R.  2013. The role of L-ascorbic acid recycling in responding to environmental stress and in promoting plant growth. Journal of Experimental Botany 64: 433-443. PMID: 23162122.

  • Cheng, S., and Gallie, D.R. 2013. Eukaryotic initiation factor 4B and the poly(A)-binding protein bind eIF4G competitively. Translation 1:1, 1-13.

  • Gallie, D.R. 2013. L-Ascorbic acid: a multifunctional molecule supporting plant growth and development. Scientifica 2013: 1–24. PMID: 24278786.

  • Cheng, S., Liu, R., and Gallie, D.R. 2013. The unique evolution of the programmed cell death 4 protein in plants. BMC Evolutionary Biology 13(1):199, 1-16. PMID: 24041411.

  • Tiruneh BS, Kim BH, Gallie, D.R. Roy B, and von Arnim AG. 2013. The global translation profile in a ribosomal protein mutant resembles that of an eIF3 mutant. BMC Biology 11(1):123. PMID: 24377433.

  • Gallie, D.R. 2013. Increasing vitamin c content in plant foods to improve their nutritional value – successes and challenges. Nutrients 5: 3424-3446. PMID: 23999762.

  • Chen Z, Jolley B, Caldwell C, Gallie D.R. 2014. Eukaryotic translation initiation factor eIFiso4G is required to regulate violaxanthin de-epoxidase expression in Arabidopsis. J. Biol. Chem. PMID: 24706761

  • Gallie, D.R. 2014. Insights from a paradigm shift: how the poly(A) binding protein bring translating mRNAs full circle. New Journal of Science. Article ID 873084.

  • Gallie, D.R. 2014. The role of the poly(A) binding protein in the assembly of the cap-binding complex during translation initiation in plants. Translation 2:2, e959378.



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