The Department of Biochemistry's weekly BCH 252 seminar series is presented this week by:

Dr. Eckhard Jankowsky, Professor,  Case Western Reserve University  

Seminar Title: "The kinetic landscape of protein binding at individual RNA sites in cells"

Abstract: The kinetics by which RNA binding proteins (RBPs) interact with their cellular RNA sites are critical for the biological function of RBPs, but it has not been possible to measure these kinetic parameters in cells. We introduce a new approach to determine transcriptome-wide kinetic parameters of protein binding to individual RNA sites in cells. The approach combines time-resolved, laser-induced, multi-photonic RNA-protein crosslinking with Immunoprecipitation, Next Generation Sequencing, and large scale kinetic modeling, to determine transcriptome-wide kinetic parameters of protein binding to individual RNA sites in cells. We also show how these kinetic data link RNA binding patterns to biological RBP function and thereby facilitate the development of predictive, mechanistic models for cellular RNA-protein interactions.

Faculty Host: Dr. Sean O'Leary; seano@ucr.edu